Research Fields of KIST Professors

research fields list
Name Lee, Cheolju
Position(full time/adjunct) Full time Professor
KIST School Major Bio-Medical Science and Technology
Division / Center Biomedical Research Institute / Center for Theragnosis
Email clee270@kist.re.kr
Telephone +82-2-02-958-6788


Research Field & Research Interest
  • Biochemistry, Protein Biochemistry, biophysics, proteomics, mass spectrometry


Research Project
  • Discovery and validation of protein signatures specific to never-smoker lung cancer
  • Analysis of proteomes and therapeutic proteins originated from stem cells
  • Discovery and validation of reverse-aging protein in blood of exercised animal model
  • Discovery and validation of reverse-aging blood protein using animal model
  • Commercialization of blood-based diagnostic kits for age-related macular degeneration
  • Development of a clinically applicable blood-based gene-protein multi-marker system enabling the diagnosis and treatment prognosis prediction of age-related macular degeneration patients
  • Protein electrofingerprinting
  • Development of N-terminome and targeted profiling proteogenomics
  • Nanowire-cell fusion bio-processor
  • Discovery of lung cancer biomarker by proteomics
  • Proteomic study on stemness marker of gastric cancer stem cells
  • Proteomics-based analysis of PPI and PTM in ARS complex
  • Discovery of biomarkers for diagnosis and monitoring of colon and breast cancers
  • Proteomics-based cancer target development
  • Discovery of cancer-specific glycoprotein by glyco-coupling
  • Development of enrichment technology of post-translationally modified proteome and quantitative proteomics for efficient biomarker discovery
  • Identification of novel receptor for immunoactive peptide from insect
  • Development of high-throughput protein production technology
  • DEVELOPMENT OF TECHNIQUES ON HIGHTHROUGHPUT PROTEIN EXPRESSION ANDPURIFICARION


Paper Publication
Paper Publication
Title Author Journal Date Abstract
Analysis of yeast proteome differentially expressed during accumulation of misfolded proteins 이철주, American Society for Biochemistry and Molecular Biology 정기 학술회의 200406
Analysis of yeast proteome differentially expressed during accumulation of misfolded proteins 이철주, IISc-KIST joint symposium 200410
Kinetic mechanism of protease inhibition by α1-antitrypsin 강운범,백제현,류승현,김준,유명희,이철주, Biochemical and Biophysical Research Communications 200410
Redox regulation of OxyR requires specific disulfide bond formation involving a rapid kinetic reaction path 이철주,Soon Mi Lee,Partha Mukhopadhyay,Seung Jun Kim,Sang Chul Lee,안우성,유명희,Gisela Storz ,류성언, NATURE STRUCTURAL & MOLECULAR BIOLOGY 200412
Protein folding and diseases 이철주,유명희, Journal of biochemistry and molecular biology 200505
ATP-의존성 단백질분해효소의 기질단백질 분해 메커니즘 이철주, 생화학분자생물학뉴스 (구-생화학뉴스) 200509
Misfolding-assisted selection of stable protein variants using phage displays 신종식,류승현,이철주,유명희, Journal of biochemistry and molecular biology 200601
Differential proteome analysis of human colorectal cancer by two-dimensional difference in gel electrophoresis and tandem mass spectrometry 김혜정,강현주,김호근,이승택,유명희,이철주, KSBMB (한국생화학분자생물학회) 200603
Quantiative proteomics analysis for PTEN signaling by stable isotope labling with amino acids in cell cylture (SILAC) 안영희,강운범,홍석호,이승록,이철주, Korean Society for Biochemistry and Molecular Biology 200605
PTEN signaling Analysis by SILAC proteomics method 안영희,강운범,홍석호,김익영,이승록,이철주, Gordon Research Conferences 200606
Rhodococcus sp. strain TM1 plays a synergistic role in the degradation of piperidine by Mycobacterium sp. strain THO100 김용학,강운범,Kyoko Konishi,이철주, Archives of microbiology 200609
Proteomic analysis of γ-butyrolacton-treated mouse thalamus reveals dysregulated proteins upon absence seizure 류명점,김대수,강운범,김준,신희섭,이철주,유명희, HUPO annual conference 200610
Quantitative profiling and biomarker in breast cancer plasma 이철주,강운범,신혁재,노동영,유명희, HUPO (Human Proteome Organization) 200610
Proteomics-based identification of breast cancer secretome for the discovery of new serological biomarkers 장종욱,강운범,김동현,이종원,노동영,이철주,유명희, 한국분자세포생물학회(KSMCB) 200610
Alloferon potentiates immune cells by down-regulating antioxidant proteins and IkBa 류명점,홍석호,유연규,전혜성,유명희,Chernysh S,이철주, AOHUPO 200612
Differential profiling of breast cancer plasma proteome by isotope-coded affinity tagging method 강운범,배지연,이재교,신혁재,이종원,김준,노동영,유명희,이철주, AOHUPO (Asia Oceania Human Proteome organization) 200612
Gel-based discovery of new serological biomarkers for breast and colon cancers 이철주, International Cancer Biomarker Consortium 200612
Identification of binding proteins for an immunoactive peptide alloferon by mass spectrometry coupled with stable isotope labeling 홍석호,안영희,강운범,유순지,이철주, 한국생물물리학회 200706
Maggot immunity and drug develop Chernysh S.,Gordja N,Chernysh N,Tulin D,Anikin V,Pleskach V.,류명점,이철주, VII International conference on biotherapy 200706
Alloferon potentiates immune cells by down-regulating antioxidant proteins and IkappaBalpha 류명점,Anikin V,홍석호,전혜성,유명희,Chernysh S,이철주, 32nd FEBS Congress 200708
Proteomic analysis of γ-butyrolactone-treated mouse thalamus reveals dysregulated proteins upon absence seizure 류명점,김대수,강운범,김준,신희섭,이철주,유명희, Journal of neurochemistry 200708
A proteomic approach to investigate potential biomarkers differentially expressed proteins between breast cancer tissues and normal tissues 김동현,노동영,유명희,이철주, HUPO 6th Annual World Congress 200710
Discovery of New Serological Biomarkers for Colon Cancers by Proteomic Approach 김혜정,이철주, 대한병리학회 200710
Identification of adenosylhomocysteinase as a novel serum tumor marker for colorectal cancer 김혜정,강현주,이한나,김동현,이승택,김호근,유명희,이철주, HUPO 6th Annual World Congress 200710
Proteomic analysis of stargazer mutant mice identified neuronal proteins involved in absence seizure 류명점,이철주,김준,신희섭,유명희, HUPO 6th Annual World Congress 200710
Optimization of expression conditions for soluble protein by using a robotic system of multi-culture vessels 안우성,안지영,정찬헌,황광연,김은경,김준,임하나,김진오,유명희,이철주, Journal of microbiology and biotechnology 200711
Identification of circulating endorepellin LG3 fragment: Potential use as a serological biomarker for breast cancer 장종욱,강운범,김동현,이재교,이종원,노동영,이철주,유명희, Proteomics Clinical applications. 200801
Validation of multiplex protein markers in breast cancer tissue by multiple reaction monitoring using the metabolically labeled concatenated standard peptides 이철주,안지영,김동현,이종원,강운범,노동영,김영수,김호근,조제열,유명희, ICBC (International Cancer Biomarker Consortium) 200802
Proteomic analysis of stargazer mutant mouse neuronal proteins involved in absence seizure 유명점,이철주,김준,신희섭,유명희, Journal of neurochemistry 200803
Analysis of Phosphopeptides from Breast Cancer Cell line MCF7 through Improved Titanium Dioxide Enrichment method 이철주,카비르, The 65th KSBMB Annual Meeting & KSMBMB-KSBMB Joint Symposium, Korea 200805
Cancer biomarker discovery through differential proteomics 이철주, KHUPO 8th Annual International Proteomics Conference 200805
Activation of NF-κB by alloferon through down-regulation of antioxidant proteins and IκBα 유명점,Vadim Anikin,홍석호,전혜성,유연규,유명희,Sergey Chernysh,이철주, Molecular and cellular biochemistry 200806
The tumour suppressor PTEN mediates a negative regulation of E3 ubiquitin-protein ligase Nedd4 안영희,황채영,이승록,권기선,이철주, The Biochemical journal 200806
바이오마커 연구개발동향 이철주, 생화학분자생물학뉴스 (구-생화학뉴스) 200806
Analysis of serum glycoproteins relevant to breast cancer by an indirect approach using Hs578T cells 안영희,강운범,유명희,이철주, HUPO 7th Annual World Congress 200808
Noninvasive molecular biomarkers for the detection of colorectal cancer 김혜정,유명희,김호근,변종회,이철주, BMB REPORTS 200810
Proteomic analysis of breast cancer tissue reveals upregulation of actin-remodeling proteins and its relevance to cancer invasiveness 김동현,배진희,이종원,김선영,김용학,배지연,이재교,유명희,노동영,이철주, Proteomics Clinical applications. 200901
Identification of S100A8 and S100A9 as serological markers for colorectal cancer 김혜정,강현주,이한나,이승택,유명희,김호근,이철주, Journal of proteome research 200903
Metastablility of native form of α1-antitrypsin and its role in functional regulation 이철주,유명희,백제현,강운범, 대한화학회 제103회 총회 및 학술발표회 200904
A proteomics approach to identify the proteins involved in FADD-DED mediated cell death in Escherichia coli 소레누르,이진희,이성기,조성원,김용학,김기선,이철주, 한국생물물리학회 2009 정기학술대회 200906
Application of MRM and mTRAQ Labeling to the Verification Process of Candidate Biomarkers Discovered by Cleavable ICAT Reagent 강운범,안영희,김경곤,이종원,김용학,신병희,김상화,김준,유명희,김영수,이철주, American Society for Mass Spectrometry 200906
단백질체학과 질량분석 유명희,이철주, 화학세계 200910
질량분석기를 이용한 단백질체의 정량분석 이철주,염정훈, 화학세계 200910
Localization of the death effector domain of fas-associated death domain protein into the membrane of Escherichia coli induces reactive oxygen species-involved cell death 소레누르,이진희,이성기,조성원,김용학,김기선,이철주, Biochemistry 201002
Mining of serum glycoproteins by an indirect approach using cell line secretome 안영희,강운범,김준,이철주, Molecules and cells 201002
Differential profiling of breast cancer plasma proteome by isotope-coded affinity tagging method reveals biotinidase as a breast cancer biomarker 강운범,안영희,이종원,김용학,김준,유명희,노동영,이철주, BMC cancer 201003
Quantitative analysis of mTRAQ-labeled proteome using full MS scans 강운범,염정훈,김호근,이철주, Journal of proteome research 201007
Interaction of T-type calcium channel Ca(V)3.3 with the β-subunit 배진희,서의진,이철주, Molecules and cells 201009
A proteomic approach based on multiple parallel separation for the unambiguous identification of an antibody cognate antigen 문주희,김용학,유종한,배진희,노동영,유명희,이철주, Electrophoresis 201010
High-throughput peptide quantification using mTRAQ reagent triplex 윤주영,염정훈,이희범,김규태,나승진,박근수,백은옥,이철주, BMC bioinformatics. [electronic resource] 201102
Comparative phosphoproteome analysis reveals more ERK activation in MDA-MB-231 than in MCF-7 Mohammad Humayun Kabir,서의진,이철주, International journal of mass spectrometry 201201
Comparative profiling of plasma proteome from breast cancer patients reveals thrombospondin-1 and BRWD3 as serological biomarkers 서의진,Mohammad Humayun Kabir,강운범,이종원,유종한,노동영,이철주, Experimental & molecular medicine 201201
Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer 정지한,김혜정,염정훈,유창영,신지혜,유진영,강창숙,이철주, Journal of gastroenterology 201201
Proteomic approach reveals FKBP4 and S100A9 as potential prediction markers of therapeutic response to neoadjuvant chemotherapy in patients with breast cancer 양원석,문형곤,김희성,최의주,유명희,노동영,이철주, Journal of proteome research 201202
Expression profiling of more than 3500 proteins of MSS-type colorectal cancer by stable isotope labeling and mass spectrometry 강운범,염정훈,김혜정,김호근,이철주, JOURNAL OF PROTEOMICS 201206
Profiling of differentially expressed proteins in stage IV colorectal cancers with good and poor outcomes 김혜정,강운범,정지한,이승택,유명희,김호근,이철주,이한나, JOURNAL OF PROTEOMICS 201206
Multiple reaction monitoring of multiple low-abundance transcription factors in whole lung cancer cell lysates 김준석,이영주,이민영,신지혜,한정민,양은경,유명희,김성훈,황대희,이철주, Journal of proteome research 201306
Reinvestigation of Aminoacyl-TRNA Synthetase Core Complex by Affinity Purification-Mass Spectrometry Reveals TARSL2 as a Potential Member of the Complex 김규태,박성준,나승진,김준석,최형원,김윤기,백은옥,이철주, PLoS ONE 201312
Quantitative proteome analysis of age-related changes in mouse gastrocnemius muscle using mTRAQ 황재영,김규태,최정이,반영재,이승민,김윤기,이철주,권기선, Proteomics 201401
Both targeted mass spectrometry and flow sorting analysis methods detected the decreased serum apolipoprotein e level in Alzheimer's disease patients 유명희,이철주,김준석,양은경,한선호,최희선 ,이영주,김종원,나덕률,황대희,묵인희, Molecular & cellular proteomics : MCP 201402
Detection and quantification of plasma amyloid-beta by selected reaction monitoring mass spectrometry. 김준석,안희성,조수민,이지은,김영수,이철주, Analytica chimica acta 201408
Discovery of melanotransferrin as a serological marker of colorectal cancer by secretome analysis and quantitative proteomics 신지혜,김혜정,김가민,송미영,우세준,이승택,김호권,이철주, Journal of proteome research 201411
Identification of vinculin as a potential plasma marker for age-related macular degeneration 김혜정,우세준,서의진,안지윤,박지현,홍혜경,이지은,안성준,황덕진,김기웅,박규형,이철주, Investigative ophthalmology & visual science 201411
Impact of data-dependent exclusion list based mass spectrometry on label-free proteomic quantification 염정훈,Kabir Mohammad Humayun,이철주, Rapid communications in mass spectrometry : RCM 201501
TRFM of Diagnostic Colorectal Cancer Marker Identified through Cell Secretome and Tissue Proteome 신지혜,김혜정,김가민,송미영,우세준,이승택,김호근,이철주, 2015 KSBMB 동계학술대회 201501
Analysis of multi-tRNA synthetase complex by mass spectrometry 박성준,안희성,이철주, KHUPO 15th Annual International Proteomics Conference 201503
Efficient separation of C-terminal tryptic peptide of amyloid-beta by reversed-phase chromatography 주신영,이진원,이철주, KHUPO 15th Annual International Proteomics Conference 201503
Impact of data-dependent exclusion list based MS on label-free proteome quantification Kabir Mohammad Humayun,염정훈,이철주, KHUPO 15th Annual International Proteomics Conference 201503
Use of Composite Protein Database for Precise Mass Spectrometric Analysis of Cell Secretome 신지혜,김가민,Kabir Mohammad Humayun,박성준,이승택,이철주, KHUPO 15th Annual International Proteomics Conference 201503
Use of composite protein database including search result sequences for mass spectrometric analysis of cell secretome 신지혜,김가민,Kabir Mohammad Humayun,박성준,이승택,이철주, PLoS ONE 201503
Proteogenomic analysis for protein-level evidence of nonsynonymous variants with gastric cancer cell line 염정훈,임병호,Kabir Mohammad Humayun,안희성,김선영,이철주, 2015 KSBMB International Conference 201505
Quantification of functional modules in gastric cancer protein networks using multiple reaction monitoring mass spectrometry 안희성,김준석,김민형,양은경,유명희,황대희,이철주, 2015 KSBMB International Conference 201505
mTRAQ-based quantitative analysis combined with peptide fractionation based on cysteinyl peptide enrichment 염정훈,강민정,신동윤,송현규,이철주,이지은, Analytical biochemistry 201505
Discovery of melanotransferrin as a serological marker of colorectal cancer by secretome analysis and quantitative proteomics 신지혜,안희성,김가민,송미영,우세준,이승택,김호근,이철주, 2015 KHUPO 하계 심포지엄 201507
Use of composite protein database including search result sequences for mass spectrometric analysis of cell secretome 신지혜,김혜정,강운범,염정훈,Kabir Mohammad Humayun,김호근,이철주, 2015 KHUPO 하계 심포지엄 201507
Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography 이철주,박성준,안희성,김준석, PLoS ONE 201511
NME1L Negatively Regulates IGF1-Dependent Proliferation of Breast Cancer Cells 유동주,Sunam Mander,박초롱,구옥재,이철주,오성현,안규리,성재영,황종익, Journal of cellular biochemistry 201601
Identification of ganglioside GM2 activator playing a role in cancer cell migration through proteomic analysis of breast cancer secretomes 신지혜,김가민,이종원,이지은,김우석,유종한,이승택,김호근,이철주,안세현, Cancer Science 201606
Proteomics-based identification and validation of novel plasma biomarkers phospholipid transfer protein and mannan-binding lectin serine protease-1 in age-related macular degeneration 김혜정,안성준,우세준,홍혜경,서의진,안지윤,박지현,유나경,이지은,김기웅,박규형,이철주, Scientific Reports 201609
A proteogenomic approach for protein-level evidence of genomic variants in cancer cells 염정훈,Kabir Mohammad Humayun,임병호,안희성,김선영,이철주, Scientific Reports 201610
Comprehensive analysis of human protein N-termini enables assessment of various protein forms 염정훈,주신영,YunJin Choi,백은옥,이철주, Scientific Reports 201707
Integrative analysis for the discovery of lung cancer serological markers and validation by MRM-MS 신지혜,Sang-Yun Song,안희성, Byung Chull An,Yoo-Duk Choi,양은경,Kook-Joo Na,Seung-Taek Lee,Jae-Il Park,Seon-Young Kim,이철주,Seung-Won Lee, PLoS ONE 201708


Patent
Patent
Subject Registration No. Date Country
Blood markers for the selection of adjuvant chemotherapy affecting patient prognosis after surgery of gastric cancer and the method using thereof Republic of Korea
Markers for blood-based diagnosis of age-related macular degeneration and diagnositic method using the same Republic of Korea
Protein marker mortalin for colon cancer diagnosis and diagnosis kit for colon cancer using antibodies against the same Republic of Korea
BCHE를 비소세포 폐암 진단용 혈액학적 마커로 사용하는 방법 10-1795595 20171102 Republic of Korea
Composition or kit comprising MFAP5 measuring agent for diagnosis of colorectal cancer and method for diagnosis using the same 20170420 Republic of Korea
Markers for blood-based diagnosis of age-related macular degeneration and diagnositic method using the same 20151116 Republic of Korea
Protein marker vinculin for diagnosis of age-related macular degeneration 20150924 United States of America
Protein marker vinculin for diagnosis of age-related macular degeneration 10-1486548 20150120 Republic of Korea
Protein marker vinculin for diagnosis of age-related macular degeneration 20140325 PCT
A PROTEIN MARKER RPE-SPONDIN FOR COLON CANCER DIAGNOSIS 10-1311718 20130913 Republic of Korea
A PROTEIN MARKERS MELANOTRANSFERRIN FOR COLON CANCER DIAGNOSIS 10-1311717 20130913 Republic of Korea
Protein markers for colorectal cancer prognosis and prognosis kit for colorectal cancer using antibodies against the same 10-1144324 20120502 Republic of Korea
A proteomic approach based on multiple parallel chromatography for the unambiguous identification of an antibody cognate antige 10-1144325 20120502 Republic of Korea
Autoantibodies for breast cancer diagnosis and multi-panel diagnosis kit using one or combination of any of the same 10-1144323 20120502 Republic of Korea
Thermostable alpha-1-antitrypsin mutein with disulfide bond 8088738 20120103 United States of America
Autoantibody against Vinculin as a biomarker for breast cancer and diagnosis kit using the same 10-1083420 20111108 Republic of Korea
A PROTEIN MARKER FIBULIN-4 FOR COLON CANCER DIAGNOSIS 20110705 Republic of Korea
PROTEIN MARKER BIOTINIDASE FOR BREAST CANCER DIAGNOSIS AND DIAGNOSIS KIT FOR BREAST CANCER USING ANTIBODY AGAINST THE SAME 10-1001275 20101208 Republic of Korea
Thermostable alpha-1-antitrypsin mutein with disulfide bond 10-0992526 20101101 Republic of Korea
A mass spectrometric method of peptide quantitation by labeling peptides with N-methylpiperazineacetic acid and its isotopologu 20100503 Republic of Korea
PROTEIN MARKER ADENOSYLHOMOCYSTEINASE FOR COLON CANCER DIAGNOSIS AND DIAGNOSIS KIT FOR COLON CANCER USING ANTIBODIE AGAINST TH 10-0883530 20090206 Republic of Korea
PROTEIN MARKER FLAVIN REDUCTASE FOR BREAST CANCER DIAGNOSIS AND DIAGNOSIS KIT FOR BREAST CANCER USING ANTIBODY AGAINST THE SAME 10-0882231 20090130 Republic of Korea
Protein marker endorepellin LG3 fragment and diagnosis kit for breast cancer 10-0847274 20080714 Republic of Korea
Thermostable alpha-1-antitrypsin mutein with disulfide bond 20071122 PCT
PROTEIN MARKERS CALGRANULIN A(S100A8) AND GALGRANULIN B(S100A9) FOR COLON CANCER DIAGNOSIS AND DIAGNOSIS KIT FOR COLON CANCER U 20070412 Republic of Korea
Centrifuge prooper to determine the stop position 0612801 20060808 Republic of Korea
Spectrometer with automatic syringe for the analysis of microorganism cultivation 20050215 Republic of Korea