The Doping Control Center was founded in the Korea Institute of Science and Technology (KIST) in 1984 to conduct doping tests for '1986 Asian Games and the '1988 Olympic Games in Seoul. As an official test laboratory accredited by the International Olympic Committee (IOC), the Center successfully performed its duties for the games. Since the establishment of the World Anti-Doping Agency (WADA) in 1999, the Center as one of WADA-accredited laboratories continues to contribute to the prevention of drug abuse in sports through research into new analytical methods for detecting newly added prohibited substances. Recently, new doping tools such as the designer steroids/stimulants, peptide hormones, blood transfusion and gene doping have been introduced to avoid detection by conventional test methods. In order to respond to these new challenges, the Center has strengthened international and local cooperative research and newly upgraded its doping control test/center system, which will significantly expand detection capabilities involving variable and unexpected situations.