Paper Abstract

Periodic nano-textured micro-patterns on intraocular lens surface to regulate posterior capsular opacity
Purpose : To identify the optimal patterning on the intraocular lens (IOL) by the synergistic effect of nano-textured micro-patterns to suppress undesirable cell migration and proliferation related to posterior capsular opacity (PCO) after cataract surgery. Methods : This experimental investigation used lens epithelial cells (LECs) and New Zealand white rabbits for in-vitro and in-vivo study, respectively. The periodic nano-textured micro-patterns were created with femtosecond laser ablation on IOL surface. The effect of both ridge (R) and groove width (G) of periodic nano-textured patterns (R5G3, R5G5, R5G10, R3G5, and R10G5) on adhesion, migration and proliferation of lens epithelial cell line (B-3) was evaluated. IOLs with the most effective pattern on its posterior surface (Group I, n=15) and without pattern (Group II, n=15) were implanted into rabbit eyes and retro-illuminated images of posterior capsule were taken at 2 months after cataract surgery. Both area and severity of PCO were measured by POCOman software in two groups. Results : In-vitro study showed that all microgroove patterns reduced the migration rate of LECs by at least 73.05%, e.g., the average migration rate of LECs on the R5G10 pattern showed the slowest between all groups (28.35μm/day, which is 93.74% smaller than the migration rate of cells in the control group). With these results, in-vivo study was performed using IOL with the R5G10 pattern. After 2 months postoperatively, both PCO area and severity in group II (35.3% and 0.44) were lower than those in group I (72.3% [P=0.001] and 1.13 [P=0.009], respectively). Conclusions : The nano-textured micro patterns on IOL may be effective to reduce the incidence of PCO after IOL implantation in cataract surgery.